منابع مشابه
Transpositional transgenesis with piggyBac
Transposons are mobile genetic elements that are capable of self-directed excision and subsequent reintegration within the host genome. Transposase such as piggyBac, Sleeping Beauty and Tol2 catalyze these reactions and have shown potential as tools for the stable integration of transgenes when used in the binary plasmid mode. Recent modifications to the transposase and/or the terminal repeats ...
متن کاملAssessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes
Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated ...
متن کاملPolynucleotidyl transfer reactions in transpositional DNA recombination.
The transposon family of mobile genetic elements is widespread among organisms. While only a handful of these elements have been analyzed biochemically, all the elements that have been studied in detail share similar reaction steps. These steps that compose the transpositional recombination process are two distinct types of polynucleotidyl transfer reactions which generate a strand transfer pro...
متن کاملTranspositional replantation of digits. Case reports.
Heterotopic or transpositional replantation of digits is technically feasible with results similar to those of conventional replantation procedures. Occasionally in multiple digital amputations not all the digits may be replanted in their correct place as a result of complex injuries proximal to the amputation zone or severe damage to important fingers. In these circumstances the amputated digi...
متن کاملProgress with schistosome transgenesis.
Genome sequences for Schistosoma japonicum and Schistosoma mansoni are now available. The schistosome genome encodes ~13,000 protein encoding genes for which the function of only a minority is understood. There is a valuable role for transgenesis in functional genomic investigations of these new schistosome gene sequences. In gain-of-function approaches, transgenesis can lead to integration of ...
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ژورنال
عنوان ژورنال: Mobile Genetic Elements
سال: 2013
ISSN: 2159-256X
DOI: 10.4161/mge.25167